Week of September 26th through October 2nd

This week I wasn't able to proceed much with the experiment. I had to order some supplies in order to clone the 3' DNA segments and have yet to receive them. In order to proceed with the 3' region of glyma02g02430.1 I designed a new primer for the 3' end and as soon as that comes in I will go ahead and use that in PCR to try and amplify that region for cloning as well. Hopefully this week I will be able to perform the 5' oligo capping and RACE in order to amplify these sequences more successfully.